Part:BBa_K106672:Design

AarI AD acceptor vector (pRS305, Gal1P, Adh1t)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2218
  Illegal EcoRI site found at 5818
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2218
  Illegal EcoRI site found at 5818
  Illegal NotI site found at 22
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2218
  Illegal EcoRI site found at 5818
  Illegal BamHI site found at 5
  Illegal XhoI site found at 924
  Illegal XhoI site found at 6629
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2218
  Illegal EcoRI site found at 5818
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2218
  Illegal EcoRI site found at 5818
  Illegal NgoMIV site found at 535
  Illegal AgeI site found at 1831
  Illegal AgeI site found at 6242
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 4254
  Illegal SapI.rc site found at 5336
  

Design Notes

The pRS305 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites.

Please note, the actual orientation of this vector is reversed.

Source

A modified pRS305 plasmid--Integrating yeast expression vector (LEU2 marker).